NAD+ protocol
NAD+ (nicotinamide adenine dinucleotide). Dosing on this page comes from a published human trial or an approved product label, cited below.
How NAD+ works
A coenzyme central to redox reactions and a substrate for sirtuins and PARPs. Tissue NAD+ falls with age, which is the basis of interest in restoring it — though whether infused NAD+ enters cells intact or is broken down first and salvaged is genuinely contested.
NAD+ dosing schedule
From a pharmacokinetic study of intravenous NAD+ in healthy men, which is the clearest published human dosing for infused NAD+ itself. Note it was designed to measure what happens to the molecule, not to demonstrate a clinical benefit.
| Phase | Amount | Frequency | Notes |
|---|---|---|---|
| Pilot study | 750 mg | Single infusion over 6 hours, intravenous | The amount and rate used in the published human PK study. |
Study length: Single 6-hour infusion in the pilot study.
Reconstitution and measurement
This part is arithmetic and does not depend on the evidence level. Concentration is vial size divided by the bacteriostatic water you add. Draw volume is your target amount divided by that concentration. Units are draw volume times 100, because a U-100 syringe reads 100 units per millilitre. Common vials for NAD+: 500 mg.
- Wipe the stoppers of both vials with an alcohol swab and let them dry.
- Draw the bacteriostatic water with a sterile syringe and inject it slowly down the inside wall of the peptide vial — never straight onto the powder.
- Swirl gently until the solution is clear. Do not shake; foaming and agitation damage peptides.
- Label the vial with the date and concentration, and refrigerate at 2–8 °C protected from light.
Worked example at the calculator defaults: 100 mg + 2 mL of bacteriostatic water = 50 mg/mL, so on a U-100 insulin syringe 1 unit = 500 mcg of NAD+. Change either input and the calculator redoes this for you.
Storage and stability
Supplies math
Whatever amounts your research uses, the planning arithmetic is the same. The calculator runs all of this per compound.
Subcutaneous technique and practical notes
General best practice from clinical injection guidance — the same fundamentals nurses are taught, none of it specific to any compound.
Reported effects
- Infusion rate is the practical constraint most often described — rapid administration is associated with flushing, chest tightness and nausea, which is why infusions are given slowly.
- The pilot study found most of the infused NAD+ appeared in urine as metabolites, raising real questions about how much reaches cells intact.
- Subcutaneous NAD+ has no comparable published pharmacokinetic study.
Sourcing NAD+
Whatever you are working with, the documentation matters more than the price. Ask for a batch certificate of analysis whose lot number matches the vial you actually receive — a COA for a different batch tells you nothing about yours.
NAD+ at Summit Research Supply → Grade a COA first
Summit is the supplier we feature and an affiliate link. Our COA checklist applies the same way to any vendor.
