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Reconstitution + Units

TB-500 Reconstitution & Dosage Calculator

This free TB-500 calculator turns your vial size, the bacteriostatic water you add, and your target amount into a concentration, a draw volume, and the exact units on a U-100 insulin syringe. TB-500 is usually measured in milligrams, which can create larger draws — the calculator flags when an amount exceeds your syringe.

Quick summary

  • Converts vial size (mg), bacteriostatic water (mL), and a milligram amount into concentration, draw volume, and U-100 units.
  • Built for milligram TB-500 amounts, with reference math for 2, 5, and 10 mg research vials.
  • Educational measurement tool only — it does not diagnose, treat, or recommend an amount.
Compound
TB-500 (Thymosin Beta-4 fragment)
Tool type
Reconstitution + unit calculator
Common research vials
2, 5, 10 mg
A common mix
5 mg + 2 mL = 2.5 mg/mL
Regulatory status
Research-use peptide; not FDA-approved for injection
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TB-500 reconstitution calculator

Step 1

Syringe

U-100 insulin
Step 2

Peptide in vial

Step 3

Target amount

Step 4

Bacteriostatic water

Volume to draw
Concentration
Draw volume
Doses / vial

Email this result to yourself

What this TB-500 calculator does

This calculator does one job well: it turns your vial size, the amount of bacteriostatic water you add, and your target amount into a concentration (mg/mL), a draw volume (mL), and the matching units on a U-100 insulin syringe. Change any input and the result updates instantly.

TB-500 ships as a freeze-dried powder. Before it can be measured into a syringe it has to be reconstituted — dissolved in bacteriostatic water. How much water you add sets the concentration, and the concentration sets how many units each amount works out to. The presets above cover the most common TB-500 vial setups; use the custom fields for anything else.

Research-use only — not medical advice. This page and calculator are educational measurement and research-planning tools. They do not recommend an amount, diagnose, or treat anything. Products referenced are sold strictly as research chemicals and are not for human or veterinary use.

How to use the TB-500 calculator

Pick your syringe

Choose the U-100 insulin syringe you'll draw with. Smaller syringes (0.3 mL / 30u) have finer lines, which helps when the draw is small.

Enter your vial and water

Set the milligrams in your TB-500 vial and the bacteriostatic water you added. Together these set the concentration.

Set your target amount

Toggle mg or mcg and pick (or type) the amount you're measuring for. The calculator does the conversion for you.

Read the draw

The result panel shows concentration, draw volume, and the exact U-100 units to pull, plus how many doses your vial contains.

TB-500 reconstitution math, explained

The math is short. Concentration = vial size ÷ bacteriostatic water. Draw volume = target amount ÷ concentration. Units = draw volume × 100 (a U-100 syringe reads 100 units per mL). The table below shows common TB-500 setups and the units for a 1 mg amount at each.

Vial sizeBac waterConcentrationUnits per 1 mg
2 mg1.0 mL2 mg/mL50 units
5 mg2.0 mL2.5 mg/mL40 units
5 mg2.5 mL2 mg/mL50 units
10 mg2.0 mL5 mg/mL20 units
10 mg3.0 mL3.33 mg/mL30 units

TB-500 amount-to-units reference

How common amounts convert to U-100 syringe units at two example concentrations. These are arithmetic conversions for reference, not a recommendation of any amount.

Units at 2.5 mg/mL (5 mg + 2 mL)
AmountVolume (mL)U-100 units
1 mg (1000 mcg)0.4 mL40 units
2 mg (2000 mcg)0.8 mL80 units
2.5 mg (2500 mcg)1 mL100 units
5 mg (5000 mcg)2 mL200 units
Units at 5 mg/mL (10 mg + 2 mL)
AmountVolume (mL)U-100 units
1 mg (1000 mcg)0.2 mL20 units
2 mg (2000 mcg)0.4 mL40 units
2.5 mg (2500 mcg)0.5 mL50 units
5 mg (5000 mcg)1 mL100 units

Mixing, color & storage tips

Milligram amounts, larger draws

TB-500 is dosed in milligrams, so draws are larger than microgram peptides. If a draw exceeds your syringe, add less water for a higher concentration, or split it across two pulls — the calculator warns you automatically.

A clear solution

TB-500 reconstitutes to a clear, colorless liquid. Discard the vial if it turns cloudy, discolored, or shows particles.

Loading vs maintenance

Some research references discuss a larger initial phase and a smaller ongoing phase. The calculator does not recommend a schedule — enter whatever amount you are measuring for and it returns the draw.

Storage

Refrigerate the reconstituted vial, keep it dark, and use it within a limited window. Keep the dry powder cold for longer storage; do not freeze a mixed vial.

TB-500 supplies checklist

A simple reconstitution shopping list. Confirm vial size and batch documentation before you buy.

TB-500 research vial
COA-verified, third-party tested
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Bacteriostatic water
0.9% benzyl alcohol, for reconstitution
Shop →
U-100 insulin syringes
0.3–1.0 mL, for accurate small draws
Any pharmacy
Alcohol prep pads
Sterilize the stopper before each draw
Any pharmacy
Verified USA Supplier
TB-500 research vial
Summit Research Supply Verified

TB-500

  • Batch COA on every vial
  • Third-party purity tested
  • U.S. fulfillment, discreet shipping
Shop TB-500 →

TB-500 — frequently asked questions

What does this TB-500 calculator tell me?

It converts your vial size, bacteriostatic water volume, and target amount into concentration (mg/mL), draw volume (mL), and U-100 syringe units.

Is the TB-500 reconstitution calculator free?

Yes — free, browser-based, no signup.

How much bacteriostatic water for a 5 mg TB-500 vial?

A 5 mg vial with 2 mL of bacteriostatic water makes 2.5 mg/mL. Because TB-500 amounts are in milligrams, using less water keeps the draw within a single syringe.

How many units is 2 mg of TB-500?

At 2.5 mg/mL it is 80 units (0.8 mL). At 5 mg/mL it is 40 units. Larger milligram amounts can exceed a 1 mL (100u) syringe, so the calculator flags that.

Is TB-500 FDA-approved?

No. TB-500 is sold as a research-use-only compound and is not an FDA-approved drug.

Is this medical advice?

No. This page and calculator are for education and research planning only. Products referenced are sold strictly as research chemicals and are not for human or veterinary use.

For laboratory research use only. PepGuru is an educational tool for measurement, dilution, and reference. TB-500 and other compounds referenced are sold strictly as research chemicals and are not for human or veterinary use, not drugs, and not intended to diagnose, treat, cure, or prevent any disease. Nothing here is medical advice. Affiliate disclosure: PepGuru features Summit Research Supply as a verified supplier and may earn a commission from purchases made through supplier links on this site.
Summit Research Supply
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